The invention relates to a cell culture flask with a supporting device. The cell culture flask comprises a flask body, a flask neck and a supporting device. The flask body is formed by four side walls, wherein every two side walls are opposite to each other. Each side wall comprises a front side wall and a back side wall opposite to the front side wall, wherein the flask neck is inclined towards one side of the front side wall, and the opening in the upper end of the flask neck is distributed in an upwards-inclined mode; the supporting device comprises a supporting plate, a plurality of sliding tables distributed in the length direction of the top face of the supporting plate, stand columns, movable sleeves and U-shaped rotating connecting bases, a counterweight base is fixedly arranged at the bottom end of the supporting plate, the sliding tables are connected to the top face of the supporting plate in a sliding mode, and a positioning bolt is in threaded connection with each sliding table; the stand columns are perpendicularly fixed to the sliding tables respectively, the stand columns are sleeved with the movable sleeves, and a fastening bolt is in threaded connection with each movable sleeve; the rotating connecting bases are fixed to the movable sleeves; rotating connecting blocks corresponding to the rotating connecting bases are fixedly arranged on the back side walls of the flask body, and the rotating connecting blocks can be connected to the interior of the rotating connecting bases in a vertical-rotation mode. The cell culture flask is convenient to operate and multifunctional.