The invention belongs to the field of traditional Chinese medicines and medicinal chemistry, and particularly relates to a mango peel extract and a preparation method and application thereof. The weight percentage of general flavones in the mango peel extract is 20 to 32 percent, and the mango peel extract is prepared by the following steps: adding an ethanol aqueous solution into a mango peel raw material for heating and reflux extraction, filtering, combining, concentrating and re-filtering an extracting solution, enabling the extracting solution to pass through macroporous resin, washing the resin with water, and abandoning water eluent; eluting with ethanol aqueous solution, collecting ethanol-containing eluent, and performing vacuum concentration, drying and smashing to obtain the mango peel extract. The mango peel extract disclosed by the invention has the effects of resisting oxidization, expanding blood vessels, reducing serum cholesterol, increasing the flow of coronary artery, resisting arrhythmia and the like, and can be used for preparing a medicine for treating cardiovascular and cerebrovascular diseases.