The invention provides a preparation device for a planting matrix of pseudo-ginseng. According to the preparation device, the problems in the prior art are solved. The preparation device comprise a feeding device (5), a raw material conveying device (301), a mixing device (3), a mixed material conveying device (302) and a screening machine (1), wherein a fine material conveying device (2) and a coarse material conveying device (4) are arranged at a discharging hole of the screening machine (1); the screening machine (1) comprises an upper-layer screening part and a pedestal part (108), and the pedestal part (108) is of a wedge-shaped structure; the upper-layer screening part comprises an isolation plate (101), a side plate (102), a screening net (104), a bottom end isolation plate (105) and a vibration machine (107), and the isolation plate (101), the screening net (104) and the bottom end isolation plate (105) are sequentially arranged on an oblique plane of the pedestal part (108) from top to bottom and are supported by the side plate (102); a spring (103) is arranged between oblique planes of the side plate (102) and the pedestal part (108), and the vibration machine (107) is mounted on the side wall of the side plate (102).