The invention relates to the technical field of microorganism screening and application, and particularly provides novel lactobacillus acidophilus with a collection number of CCTCC NO: M2016429. The novel lactobacillus acidophilus obtained by screening is high in antibacterial ability, has obvious inhibition effect on salmonella, staphylococcus aureus and helicobacter pylori and especially has strongest inhibition effect on Escherichia coli, and diameter of the inhibition zone is up to 24mm. The novel lactobacillus acidophilus can be effectively bred in gastric acid and has high tolerance to bile salt. The lactobacillus acidophilus can strongly remove cholesterol, and removal rate is up to 91.4%. The strain has high anti-oxidation ability, and removal rates of fermentation supernate of the strain to DPPH.and hydroxyl radicals are up to 92.6% and 94.1% respectively. The novel lactobacillus acidophilus can be widely applied in preparation of lactobacillus oral solutions, can obviously increase number of intestinal beneficial bacteria, improve gastrointestinal tract health and improve body immunity and has wide application prospect.