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发明公开
CN106350517A 水稻高精度分子标记Xa7-SM5及其育种利用 无效 - 撤回

水稻高精度分子标记Xa7-SM5及其育种利用
摘要：
本发明公开了一种水稻Xa7基因高精度分子标记Xa7-SM5，分子标记的扩增引物选自下列引物对，正向：5’TCTAGCTACAGAATCCGATCGAAT 3’，反向：5’CCAATTAGCAATCCTGTGTACCAT 3’。其能用于含Xa7的水稻与不含Xa7的水稻之间的分子检测及其后代的标记辅助选择；当PCR扩增产物的电泳中含有如SEQ ID NO：1所示序列时，为含Xa7的水稻；反之，则为不含Xa7的水稻。
摘要（英）：

The invention discloses a high-precision molecular marker Xa7-SM5 of a rice Xa7 gene. An amplification primer of the molecular marker is selected from the following primer pair including a positive primer of 5'TCTAGCTACAGAATCCGATCGAAT 3' and a reverse primer of 5'CCAATTAGCAATCCTGTGTACCAT 3'. The high-precision molecular marker Xa7-SM5 can be used as the molecular detection between Xa7-containing rice and Xa7-free rice and the marker auxiliary selection of the later generation; when a sequence shown by SEQ ID NO:1 is contained in electrophoresis of a PCR (polymerase chain reaction) amplification product, the rice is the Xa7-containing rice; otherwise, the rice is the Xa7-free rice.

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