The invention belongs to the technical field of tobacco component detection, and particularly relates to an analysis method for realizing quantitative determination of Amadori compounds in tobacco. The analysis method comprises the following steps: 1) sample preparation: pulverizing a tobacco leaf or tobacco shred sample, and passing through a 40-mesh screen; 2) extraction: weighing the tobacco powder sample, adding a methanol/water solution, performing ultrasonic extraction, performing high-speed centrifugation on the extraction liquid, taking the supernate, and filtering; 3) dilution: diluting methanol with the filtrate to obtain a sample solution; and 4) determination: determining the sample solution in an HPLC-MS/MS (high performance liquid chromatography-mass spectrometer/mass spectrometer) MRM (multiple reaction monitoring) mode. According to the invention, the pulverized tobacco leaf/tobacco shred sample is directly extracted by the extraction solvent, centrifugated, filtered and diluted, and the determination is directly performed by means of HPLC-MS/MS, so that 10 Amadori compounds in tobacco can be simultaneously detected; and the analysis method has the advantages of simple, quick and mild operation process, favorable method reproducibility and recovery rate, high accuracy and sensitivity and multiple Amadori compound determination varieties.