The invention relates to an interplanting cultivation method of lentinula edodes, morchella vulgarises and kudzuvine roots. The interplanting cultivation method comprises the following steps: a, building a greenhouse; b, preparing open boxes; c, sowing morchella vulgaris; d, managing mushroom production; e, mounting movable lentinula edodes frames; and f, cultivating and harvesting fruit bodies. According to the interplanting cultivation method of the lentinula edodes, the morchella vulgarises are planted under the lentinula edodes frames and the lentinula edodes are planted on the frames in the greenhouse without influencing each other, thereby saving lands. Both the morchella vulgarises and the lentinula edodes need scattered light and do not influence each other. The watering management of the lentinula edodes in peacetime can supply enough water to the ground, the morchella vulgarises may not need watering to save manpower, the interplanting of the lentinula edodes and the morchella vulgarises can increase income, and kudzu vines replace traditional sunshade nets, so that a lot of expenses can be saved. The photosynthesis of kudzu leaves and the respiration action of lentinula edodes mycelia can be complementary. The transpiration of the kudzu vines can better lower the temperature, achieves a shading effect, and has an environment-friendly effect, so that the utilization rate of the greenhouse is effectively improved, and the economic value is improved.