The invention provides coregonus autumnalis sperm compound activation liquid. The sperm compound activation liquid consists of glucose and sodium chloride, wherein sodium chloride has a certain inhibiting effect on saprolegniasis in a hatching process of coregonus autumnalis fries, and glucose has a relatively good activation effect on coregonus autumnalis sperms. According to the coregonus autumnalis sperm compound activation liquid, through scientific proportioning, the usage amount of carbohydrates is reduced, meanwhile, the usage amount of salts is increased, and not only the good activation effect of carbohydrates but also the good saprolegniasis inhibiting effect of salts are utilized to realize complementation of advantages. Experiments find that if the semen is not activated by the sperm compound activation liquid, the rapid movement activation rate of sperms is 80%, and the rapid movement time of sperms lasts for 20s; and if the semen is activated by the sperm compound activation liquid, the rapid movement activation rate of sperms is over 95%, and the rapid movement time of sperms lasts for 55.2s, and thus, the sperm compound activation liquid can obviously improve the activity of coregonus autumnalis sperms.