The invention discloses a preparation method of a compound product of glossy ganoderma polysaccharide and lactic acid bacteria. The preparation method comprises the following step of compounding the prepared glossy ganoderma polysaccharide liquor with the lactic acid bacteria according to the ratio of the prepared glossy ganoderma polysaccharide liquor to the lactic acid bacteria being 10 to 1, so that the compound product is obtained. According to the preparation method disclosed by the invention, a glossy ganoderma polysaccharide extraction technique suitable for large-batch production and an optimized preparation technology of the lactic acid bacteria are adopted, and the effects of the lactic acid bacteria and the effects of the glossy ganoderma polysaccharide are combined, so that the compound product has remarkable effects in the respects of enhancing the immunity of broiler bodies and reducing the death rate, and is safe and free of side effects.