The invention discloses a pine pollen composition with a defaecation improving effect and a preparation method of the pine pollen. The composition comprises effective ingredients and auxiliary additive ingredients allowable in medicine and food, wherein the effective ingredients include, by weight, 60-200 parts of dandelion extract, 50-160 parts of sophora flower extract, 10-40 parts of bitter almond extract, 10-40 parts of lophatherum gracile extract, 4-20 parts of fructus momordicae extract, 130-350 parts of pine pollen and 130-400 parts of xylooligosaccharide. The pine pollen composition has the advantages that the ingredients are all substances used for both medicine and food, the effects of improving gastrointestinal function, regulating blood lipids and stabilizing blood sugar can be achieved while bowels are relaxed, diarrhea is not caused, and toxic and side effects are avoided.