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发明公开
CN106362212A 一种用于去除组织细胞的亲脂性去细胞溶液、试剂盒和方法 无效 - 驳回

一种用于去除组织细胞的亲脂性去细胞溶液、试剂盒和方法
摘要：
本发明涉及一种用于去除组织细胞的亲脂性去细胞溶液，其为CHAPS浓度为5-50g/L、TnBP浓度为1-5mM、ASB-14浓度为5-50g/L并且SB 3-10浓度为5-50g/L的20-100mM的TRIS-HCl缓冲液；还涉及一种用于去除组织细胞的试剂盒，其由A液和B液组成，所述A液为上述亲脂性去细胞溶液，B液为全能核酸酶溶液；还涉及一种用于去除组织细胞的去细胞方法，使用上述试剂盒处理所述组织。以上试剂和方法制备的异种组织去细胞生物支架去细胞完全，细胞外基质成分保留好，力学性能损伤小，免疫原性低，制备周期短、费用低，具有很好的临床应用前景。
摘要（英）：

The invention relates to a lipophilic decellularization solution for removing tissue cells. The lipophilic decellularization solution is a 20-100mM TRIS-HCl buffer solution containing 5-50g/L CHAPS, 1-5mM TnBP, 5-50g/L ASB-14 and 5-50g/L SB 3-10. The invention also relates to a kit for removing tissue cells. The kit is composed of a solution A and a solution B, wherein the solution A is the lipophilic decellularization solution, and the solution B is a benzonase nuclease solution. The invention also relates to a decellularization method for removing tissue cells. The decellularization method comprises the following step: treating the tissues by using the kit. The heterologous tissue decellularized biological scaffold prepared by the reagents through the method realizes full decellularization, favorably retain extracellular matrix components, has the advantages of small mechanical performance damage, low immunogenicity, short preparation period and low cost, and has favorable clinical application prospects.

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