The invention provides an ultraviolet photo-curing anti-fog coating which is composed of, by weight: 50-60 parts of hydrophilically treated silicon dioxide microspheres, 7-12 parts of hydrophilic high-molecular long-chain polyurethane acrylate, 20-30 parts of acetone, 8-12 parts of an aqueous monomer, and 0.9-1.2 parts of a photo-initiator. In the invention, the hydrophilic silicon dioxide microspheres being subjected to special surface modification is matched with the special hydrophilic high-molecular long-chain polyurethane acrylate, so that the anti-fog coating has good solvent resistance, is ultraviolet-resistant, and has excellent hydrophilicity, durable water resistance and excellent anti-fog property.