The invention belongs to the technical field of microfluidic chips, and particularly relates to an experimental facility of using the microfluidic technology to measure fluid viscosity. A constant pressure pump, a microsyringe, a micro-fluidic chip, and a waste liquor collecting system are connected in sequence, and the microsyringe is connected to the micro-fluidic chip through a teflon tube; a temperature-controlling system is connected to a glass-heating platform; a laser light source, a forwardly installed fluorescence microscope and a digital camera are connected in sequence; the digital camera is located above an objective table of the forwardly installed fluorescence microscope; the micro-fluidic chip is put on the glass-heating platform, and the glass-heating platform is put on the objective table of the forwardly installed fluorescence microscope. According to the experimental facility of using the microfluidic technology to measure fluid viscosity, quantitative change laws of the fluid viscosity in a wider temperature range can be obtained. A method is simple and practicable, has a high degree of accuracy, has a wide temperature measuring range, and has low cost, which is suitable for being used as undergraduate teaching experiments in related discipline of hydromechanics.