The invention is applicable to the technical field of an ion generator, and provides a positive and negative ion generation electrode and a positive and negative ion generator. The positive and negative ion generation electrode comprises a plasma generation electrode, a positive ion generation end which is perpendicularly arranged in a first through hole of the plasma generation electrode, and a negative ion generation end which is perpendicularly arranged in a second through hole of the plasma generation electrode; the positive ion generation end is connected with multiple first carbon fibers through a first high-voltage flexible wire; the negative ion generation end is connected with multiple second carbon fibers through a second high-voltage flexible wire; and the plasma generation electrode, the positive ion generation end and the negative ion generation end are connected with a positive and negative ion generation circuit. By adoption of the embodiments of the positive and negative ion generation electrode and the positive and negative ion generator, the problems as follows are solved: the existing ion generator only generates negative ions and has a bad sterilizing effect; meanwhile, a needle-shaped electrode is commonly adopted to be used as a negative ion generation part; and the position of the electrode part is relatively fixed, so that the electrode is inconvenient to disassemble and assemble, and the installation and use of the electrode in different occasions are inconvenient.