The invention discloses LED lamp testing and assembling equipment with an anti-vibration testing function. A turnover device is used for turning over lamp holders in the vertical state originally to be in the flat-on state; an anti-vibration testing device is used for conducting anti-vibration capacity testing on the lamp holders; a feed divider is used for turning over the lamp holder in the flat-on state to be in the vertical state again, and is used for separating the lamp holders which are next to one another; a lamp cap feeding device is used for feeding lamp caps into an assembling device and used for assembling the lamp caps with the lamp holders; and a discharging device is used for conducting discharging recycling on the assembled lamp holders and lamp caps, and therefore the testing and assembling process of products are completed. By means of the arrangement of the turnover device, the anti-vibration testing device, the feed divider, the lamp cap feeding device, the assembling device and the discharging device, the above devices are subject to structural optimization, and mechanical automation testing and assembling of LED lamps are achieved.