The invention discloses a preparation method of a nickel tungsten tetraoxide nanowire. The preparation method of the nickel tungsten tetraoxide nanowire comprises the main steps of firstly respectively preparing deionized water solutions of sodium tungstate, nickel sulfate and sodium carboxymethylcellulose, then mixing the solutions of the nickel sulfate and the sodium carboxymethylcellulose, stirring for 30min, mixing the mixed solution with a sodium tungstate solution, ultrasonic processing the mixture for 30min, and finally carrying out hydro-thermal treatment to obtain the nickel tungsten tetraoxide nanowire. The preparation method of the nickel tungsten tetraoxide nanowire provided by the invention has the advantages of simplicity in operation, controllable morphology, low cost and the like, and is easy for realizing industrial production; the prepared nickel tungsten tetraoxide nanowire has a one-dimensional structure, has a high length-diameter ratio, large specific surface area, and has wider application in the fields such as catalysts, light-guide fibers, capacitors, humidity sensitive materials, lasers, conducting materials, detectors and sensors.