The invention provides a method for determination of a fatty acid value of corn. The method comprises A, mixing a corn sample and waterless ethanol, carrying out oscillation for 9-20min, filtering the solution and collecting the filtrate, and B, adding a phenolphtalein indicator into the filtrate drop by drop, carrying out titration on the filtrate through a primary standard substance-containing potassium hydroxide standard solution and a primary standard substance-less potassium hydroxide standard solution until the filtrate is in reddish color, eliminating yellow interference through an orange filter, respectively recording consumption volumes of the potassium hydroxide standard solutions and calculating a fatty acid value of corn. The method solves the problem that the detection period is long and a terminal point is difficult to determine, realizes accurate detection of a fatty acid value of corn in short time, greatly simplifies operation, utilizes mild operation conditions, is worthy of promotion and application and provides a referenced technical route for later application.