The invention discloses an intelligent check and acceptance system of substation anti-misoperation locking logic. The system comprises an engineering management module used for performing comprehensive management on engineering information, a drawing tool module used for performing conversion, drawing and repair processing on a graph, a topology module used for performing a graph topology search based on graph information and automatically generating anti-misoperation logic according to a topology rule language, a logic formula analysis module used for analyzing a topology language and logic languages of different anti-misoperation system manufacturers, and a check result and feedback information management module used for checking a substation microcomputer anti-misoperation system logic formula according to the anti-misoperation logic generated by topology analysis. Therefore, the technical effects of capability of automatically performing check and acceptance, and relatively high efficiency and accuracy are achieved.