The invention discloses a method for establishing an erucic acid induced fish enteritis model. The method comprises the following steps: selecting normal cultured fishes as objects for establishing an enteritis model; and feeding the cultured fishes with an experimental feed in which the content of erucic acid is 12g/kg, thereby obtaining the erucic acid induced fish enteritis model. The method for establishing the erucic acid induced fish enteritis model, disclosed by the invention, has smallest influence on production performance, growth development of intestinal tracts, immunity and muscle quality of the fishes, but caused enteritis pathogenic manifestations are most suitable, and the model is stable and reliable, and has good reproducibility.