The invention discloses a medicine extract for strengthening an anti-virus capability and an immune function of a livestock, a preparation, and a preparation method. The medicine extract comprises the following raw herb materials in parts by weight: 300-900 parts of astragalus membranaceus, 100-300 parts of radix isatidis, 100-300 parts of radix saposhnikoviae, 100-300 parts of roasted rhizoma atractylodis macrocephalae, 100-400 parts of scutellaria baicalensis, and 100-500 parts of echinacea; and the invention also discloses a preparation and a preparation method. The techniques of extracting, on-line high-speed centrifugation, vacuum concentration and spray drying are used, the techniques save time and labor, the efficiency is high, the consumption is low, and effective ingredients are sufficiently kept; the scientific matching of the medicines strengthens the anti-virus capability and the immune function of the livestock; furthermore, the prepared medicine extract for strengthening the anti-virus capability and the immune function of the livestock can be prepared into soluble powder, granules, oral liquid, tablets and the like, the clinical application of the medicine extract on the livestock is implemented, the anti-virus capability and the immune function of the livestock are improved, and a better effect is achieved.