The invention relates to a fluorescent multicolor floor and a preparation method thereof. The fluorescent multicolor floor comprises a ground base layer, a ground coat and a fluorescent layer, wherein the ground coat is uniformly adhered to and covers the upper surface of the ground base layer; the fluorescent layer is adhered to and covers the ground coat. The fluorescent multicolor floor prepared according to the invention is environmental-friendly, has simple and convenient process and excellent modificability, has a fluorescent multicolor fantastic art style, also has excellent chemical resistance, excellent wear resistance, high hardness, high adhesive force, high impact resistance, low possibility of aging, simplicity in cleaning and overall seamlessness, and is applied to multiple leisure and entertainment occasions.