The invention relates to the technical field of wallpaper, in particular to anti-acarid wallpaper and a preparation method thereof. The anti-acarid wallpaper sequentially comprises a base material layer, a resin layer, an ink layer and an anti-acarid layer from bottom to top. A mould-proof and water-proof layer is arranged on the bottom face of the base material layer. The anti-acarid wallpaper has a good decoration function, meanwhile has the anti-acarid effect and is long in anti-acarid time and good in effect; the mould-proof and water-proof layer is arranged on the bottom face of the base material layer, wall face water seepage is prevented, and bacterial growth is restrained; and the preparation process is simple, and the anti-acarid wallpaper can be produced in batches and is low in cost.