The invention provides a semi-conductor refrigeration and heat dissipation component, an assembly method of the semi-conductor refrigeration and heat dissipation component and refrigeration equipment. The semi-conductor refrigeration and heat dissipation component comprises a semi-conductor refrigeration chip, a cold dissipater arranged on the cold end surface side of semi-conductor refrigeration chip and a radiator arranged on the hot end surface side of the semi-conductor refrigeration chip, and further comprises a heat insulation mounting part, wherein the cold dissipater and the radiator are arranged on two sides of the heat insulation mounting part at an interval and connected with the heat insulation mounting part. The invention solves the problem of poor refrigeration effect of refrigeration equipment in the prior art.