A measuring device for a biosensor comprises a cell recognition module, a biosensor, and a measuring apparatus. The cell recognition module is used to determine the biological species. The biosensor is inserted into the measuring apparatus and is used to quantify the matrix in a sample solution. The measuring apparatus comprises a support part of the biosensor, wherein the support part can be easily assembled or disassembled and forms at least a pair of electrodes on an insulation substrate; a plurality of connection terminals, which are electrically connected to the electrodes; and a driving power supply, which applies a voltage to the electrodes through the connection terminals. The measuring device can reduce the measurement error of the biosensor.