The invention discloses a method for anesthetizing coreius guichenoti. The method is applicable to artificially reproducing the coreius guichenoti. The method includes steps of 1), classifying specifications, to be more specific, carrying out fasting on the coreius guichenoti for 24 h, then fishing the coreius guichenoti by the aid of disinfected nets, weighing the coreius guichenoti and placing the coreius guichenoti with the weight of 110-160 g into disinfected cultivation tanks for anesthetizing the coreius guichenoti; 2), temporarily cultivating the classified coreius guichenoti for 9-11 d to allow the constitution of the coreius guichenoti to be recovered, and anesthetizing the coreius guichenoti after the coreius guichenoti can be normally fed; 3), placing the coreius guichenoti in 100-180 mg/L MS-222 solution and anesthetizing the coreius guichenoti under the condition of the water temperature of 16.1-16.5 DEG C for the anesthesia time of 86-163 s. The method has the advantages that effective anesthetic treatment is carried out on the coreius guichenoti, accordingly, strong stress response of the coreius guichenoti can be reduced, and operation for weighing, marking and artificially reproducing the coreius guichenoti and the like can be smoothly carried out in domestication procedures.