The invention provides a preparation method of a solidified tobacco extract. The preparation method comprises the following steps: 1) conducting extracting: drying, crushing and sieving tobacco raw materials, and conducting supercritical extraction; 2) conducting separating: dissolving an extract obtained in the step 1) and uniformly mixing the dissolved extract, cooling an obtained solution until the solution becomes layered, conducting filtering to remove lower liquid, and reserving an upper solid so as to obtain a primary shaped material; and 3) conducting shaping: dissolving the primary shaped material with the addition of an atomizing agent, oscillating and uniformly shaking, and condensing and shaping so as to obtain the solidified tobacco extract. Furthermore, the invention also provides an application of the solidified tobacco extract prepared by the method as an aerosol forming matrix in an electronic cigarette. According to the preparation of the solidified tobacco extract and the application of the solidified tobacco extract in the electronic cigarette provided by the invention, various problems caused by the addition of additives as well as flavors and fragrances can be avoided, and such problems as tar leakage and the like caused by conventional electronic cigarette liquid can be also avoided; therefore, the solidified tobacco extract is quite worthy of popularizing and applying.