The invention relates to an ointment and a preparation method thereof, in particular to an acne ointment and a preparation method thereof. The acne ointment comprises a morning ointment and an evening ointment. The morning ointment comprises a first ingredient and a corresponding substrate, and the evening ointment comprises a second material and a corresponding substrate. The first material comprises 2% of nicotinamide, 1% of ortho-hydroxybenzoic acid, 1% of clindamycin, 1.5% of oil soluble azone and 0.2% of Nepal methyl ester. The second material comprises 10% of anchoic acid, 0.1% of Adapalene, 1% of clindamycin, 1% of salicylic acid, 1.5% of grease azone and 0.2% of Nepal methyl ester. The ointment has the advantages of having easily sourced material, being non-toxic and safe, having good therapeutic effects and being simple in preparation.