The invention relates to a novel sterilization device for medical apparatus and instruments. The sterilization device comprises a bracket, a box body and a box cover. A box door is formed in the front side wall of the box body, a top motor is arranged on the box cover, a rotary shaft is connected with the bottom end of an outlet shaft of the top motor, a plurality of trays are uniformly arranged on the rotary shaft in a sleeving mode, and stopping barrels are arranged on the external circumference of each tray. A water storage tank is arranged on the box cover, a water inlet and an agent feeding hole are formed in the top end of the water storage tank, a first water outlet is formed in the bottom of the left side of the water storage tank, and a first water outlet pipe is connected with the first water outlet and extends downward into the top end inside the box body. A bottom motor is arranged at the bottom end outside the box body, and a plurality of stirring bars are connected with the top end of an output shaft of the bottom motor. A left heating cavity and a right heating cavity are formed in the left side and the right side of the box body respectively, and a left electric heater and a right electric heater are arranged in the left heating cavity and the right heating cavity respectively. By means of the structure, the sterilization device is more convenient and efficient to use, and meanwhile the sterilization efficiency and sterilization effect of the sterilization device are greatly improved.