The invention relates to a preparation method of a graphene-oxide-based hybrid biological functional coating. The preparation method comprises the following steps: 1, preparing a graphene oxide/nanosilver composite material; 2, treating a titanium sheet; 3, grafting the titanium sheet with the graphene oxide/nanosilver composite material; 4, preparing a graphene oxide/nanosilver/collagen hybrid coating. The preparation method has the advantages as follows: (1) by a method for reducing nanosilver by using ultraviolet, the uniformly-dispersed nanosilver with a small size can be obtained, and adverse effects on the environment are avoided; (2) on the basis of graphene oxide, graphene oxide, collagen and nanosilver are organically combined, and through coordinated action of the three components, respective shortcomings can be improved, so that the graphene-oxide-based hybrid biological functional coating has a more excellent biological function; (3) when the preparation method provided by the invention is used for preparing the graphene oxide-based hybrid biological functional coating, the equipment investment and the implementing difficulty are low; the preparation method is simple and feasible; by the preparation method, too much influence on the environment is avoided.