The invention provides a penicillin bottle handling method, a penicillin bottle obtained through handling, and penicillin-bottled water for injection, and belongs to the technical field of medicine. The penicillin bottle comprises a bottle body and a butyl rubber plug. The penicillin bottle handling method comprises the following steps in any sequence: (1) handling the bottle body by utilizing an acid solution and an alkaline solution sequentially, carrying out cleaning till the pH value reaches 5.0-7.0, and carrying out drying and sterilization for standby application; and (2) handling the butyl rubber plug by utilizing the acid solution, the alkaline solution and warm water sequentially, carrying out cleaning till the pH value reaches 5.0-7.0, and carrying out sterilization for standby application. The invention further provides a preparation method of the penicillin-bottled water for injection. The preparation method comprises the following step: filling the penicillin bottle obtained through handling with the water for injection. The water or liquid medicine for injection accommodated in the penicillin bottle obtained according to the penicillin bottle handling method, or the penicillin-bottled water or liquid medicine for injection obtained according to the preparation method not only is qualified and high in stability, but also can be protected from chloride leakage, chloride out-of-specification and the like after standing for a long time, and is suitable for long-term preservation.