The invention discloses a gift box with a music playing system. The gift box comprises a gift box body. The gift box body comprises a box cover and a box body. The box body is internally provided with a first partition and a second partition which are perpendicular to the bottom of the box body and are movably connected with the box body. The box body is further provided with a music playing system. The music playing system comprises a first microcontroller, a second microcontroller, a music play/stop button, a song switching button, a buzzer and a nixie display. According to the gift box, the gift box and the music playing function are combined, the gift box can be used for packaging a gift and can also express the mind of a sender through the song set in advance, and interaction and friendship of both sides can be enhanced; and through the arrangement of the movable partitions, the inner space of the gift box can be flexibly arranged according to the size and number of gifts, and the gift box is convenient and practical to use.