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发明公开
CN106435030A 一种联合检测禽流感H7N9和H9N2的四通道荧光定量PCR检测方法及试剂盒 有权

一种联合检测禽流感H7N9和H9N2的四通道荧光定量PCR检测方法及试剂盒
摘要：
本发明公开了一种联合检测禽流感H7N9和H9N2的四通道荧光定量PCR检测方法及其试剂盒。本发明方法能够同时检测出待测标本中禽流感病毒H7N9的H7基因和N9基因，以及禽流感病毒H9N2的H9基因和N2基因。由于该方法引入了特异性扩增引物和荧光探针，使得检测的灵敏度和特异性得到很大程度的增强，从而避免了其他检测方法特异性不高容易漏诊和误诊的问题。同时，该试剂盒根据本方法原理制得的试剂盒，可方便地同时检测出禽流感病毒H7N9的H7基因和N9基因，以及禽流感病毒H9N2的H9基因和N2基因，准确率高。
摘要（英）：

The invention discloses a four-channel fluorogenic quantitative PCR detection method and fluorogenic quantitative PCR kit for combined detection of avian influenza H7N9 and H9N2. By adopting the method disclosed by the invention, genes H7 and N9 of an avian influenza virus H7N9 and genes H9 and N2 of an avian influenza virus H9N2 in a sample to be tested can be simultaneously detected. A specific amplification primer and a fluorescent probe are introduced in the method, so that the sensitivity and specificity of detection can be enhanced to a large extent to avoid the problem of low specificity of other detection methods to easily result in missed diagnosis and misdiagnose. Furthermore, the kit is obtained according to principles of the method, so that the genes H7 and N9 of the avian influenza virus H7N9 and the genes H9 and N2 of the avian influenza virus H9N2 can be conveniently and simultaneously detected, and the accuracy is high.

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