The invention discloses a method of measuring the content of curcumin-type compounds in body of Chinese softshell turtles. The method includes the following steps: (A) extracting plasma, muscle and skin of the Chinese softshell turtles with a solution A; (B) performing evaporation drying to an extract; (C) dissolving residues after nitrogen blowing with a solution and adding a reagent; (D) sucking liquid in the lower layer and enabling the liquid to pass through a polyether sulfone aqueous filtering head to obtain a filtrate which is then placed in a bottle, and detecting the filtrate; (E) performing ultra-high performance liquid chromatographic analysis, wherein an organic phase of a mobile phase is the reagent while a water phase is a solution, setting the volume ratio of the solution to the reagent, the flow speed and column temperature, thus obtaining peak area; (F) with mass concentrations and peak areas of bisdemethoxycurcumin, demethoxycurcumin and a curcumin standard solution respectively as horizontal ordinates and vertical ordinates, drawing a standard curve; and (G) measuring the content of the compounds in the plasma, muscle and skin of the Chinese softshell turtles. The method has convenient and quick operations, short analysis time and high accuracy and sensitivity, and is suitable for quickly and accurately measuring the contents of the bisdemethoxycurcumin, demethoxycurcumin and curcumin in the tissue of the Chinese softshell turtles.