The invention discloses a method for asexual cloning and quick propagation of Shixing Dendrobium seedlings, and the method comprises the following main steps: preprocessing, inducing culture, protocrom-like body formation, protocrom-like body proliferation, differentiation culture, rooting and seedling strengthening and transplanting, and in the process, a protocrom-like body inducing culture medium is M1, a proliferation culture medium is M2, a differentiation culture medium is M3 and a rooting and seedling strengthening medium is M4. According to the method, a Shixing Dendrobium high-quality individual plant is selected, a plant tissue culture technique is used for rapid and mass propagation of high quality seedlings, and the large-scale and commercial production of the Shixing Dendrobium seedlings are facilitated. Only simple plant tissue culture equipment is used in the method for successful asexual cloning and quick and mass propagation of Shixing Dendrobium, and the method has the advantages of low cost and high efficiency.