The invention discloses a protective glove for preventing burn injury from 400 V and below, comprising a glove body, conducive cloth, a cover bag and a magnet; the glove body is made of pure cotton, the conductive cloth is sewn to the inner outer surface of the thumb part of the glove body, the glove body is provided with three or more button seams, the cover bag is a closed bag, the magnet is arranged in the cover bag, and a plurality of buttons are sewn to the outer surface of the cover bag. The protective glove may protect staff from arc burn injury possibly due to circuit shorting and electrical equipment aging when the staff take off the protective gloves and test electricity of electrical equipment, protect from electric shock due to the mistaken contact with electrified circuit equipment when staff approach the electrified equipment, and decrease the injury of staff due to potential safety hazards.