The invention discloses water-soluble fertilizer containing amino acid prepared with an enzymolysis method and a preparation method of the water-soluble fertilizer. The water-soluble fertilizer containing amino acid and microelements is produced by dissolving and chelating raw materials including superior amino acid prepared with the enzymolysis method, chelated copper-zinc-iron-manganese, boric acid and ammonium molybdate in a reaction kettle. The foliage spray water-soluble fertilizer has the advantages that the fertilizer combines organic and inorganic matter, contains comprehensive microelements and contains balanced nutrition, the crop yield is increased, and the crop quality is improved.