The invention provides a yellow reactive dye composition and a dyeing application on fibers and a method for applying the yellow reactive dye composition to dyeing the fibers. The yellow reactive dye composition comprises a type of or a plurality of types of dye compounds shown as a general formula (I) and a type of or a plurality of types of dye compounds shown as a general formula (II). The dye compounds are mixed with one another according to certain proportions. The yellow reactive dye composition, the application and the method have the advantages that the yellow reactive dye composition is excellent in different types of fastness and is particularly excellent in light fastness and wet-treatment fastness and can be widely used for coloring cellulose fibers, polyamide fibers, protein fibers, blended fabrics and the like, and extraordinary coloring effects can be realized when the yellow reactive dye composition is used for carrying out tone-on-tone dyeing on nylon cotton blended fabrics by the aid of one-bath one-step processes.