A wheat stalk breaking strength molecular marker is disclosed, the wheat stalk breaking strength molecular marker is located between wheat 4B chromosome TDURUM-CONTIG63670-287 and IACX557, and named as QWQD4B.4-13; through application of the molecular marker, whether QWQD4B.4-13 gene loci for increasing stalk breaking strength exist in wheat varieties or lines can be detected, the breeding process of lodging-resistant wheat varieties can be accelerated, by use of the special stalk breaking strength molecular marker, the screening is fast and accurate, and is not affected by the environment, a target is clear to select, cost of production is saved, the selection efficiency and quality of the lodging-resistant high yield wheat varieties or lines are greatly improved, QTL detection effect and precision can be improved, the effective molecular marker is developed, a stalk breaking strength molecular assisted breeding system is developed, and the stalk breaking strength molecular assisted breeding system can be programmatically applied in breeding practices.