The invention discloses an automatic bacterium applying system for beauveria bassiana production. The automatic bacterium applying system comprises an upper sliding track, a bacterium applying device, a controller and a signal acquisition module, wherein the upper sliding track is arranged on the ground inside a silkworm house; the bacterium applying device is arranged on the upper sliding track; the controller is arranged outside the silkworm house; the signal acquisition module is arranged inside the silkworm house; the bacterium applying device is enabled to walk on the upper sliding track through a driving device arranged at the bottom; the upper sliding track extends out of the silkworm house; the driving device is in signal connection with the controller; the signal acquisition module is used for transmitting a signal to the controller; the controller is used for outputting a control signal to the driving device. By adopting the automatic bacterium applying system, the bacterium applying degree can be judged according to parameters such as the temperature, the humidity and the spore concentration of silkworm bases in the silkworm house, walking control on the bacterium applying device can be achieved according to the bacterium applying degree, automatic adjustment can be completed, uniform bacterium application can be achieved, pollution and influence to the environment of the silkworm house can be reduced while operators get in and out of the silkworm house, the manual operation can be reduced, the production efficiency can be improved, and the large-scale automatic production of silkworm larvae can be achieved.