The invention discloses a rapid detection method of a DNA amplification product. The rapid detection method comprises the following steps: respectively adding different Tag sequences on 5' ends of upstream and downstream primers of a target DNA; modifying fragments between the primers and Tag by using an aromatic compound with two hydroxymethyl groups, so as to enable the PCR amplification product to have terminal single-chain Tag; respectively designing sequences complementary with Tag of the upstream and downstream primers, and respectively binding the complementary sequences onto colloidal gold particles to produce a marked colloidal gold solution; and mixing the PCR amplification product with the marked colloidal gold solution, and judging the detection result visually, without the need of other instruments and equipment, so that the detection cost is greatly saved, and the rapid detection method has high detection sensitivity and wide universality.