The invention discloses a clostridium perfringens dry powder LAMP rapid detection kit and a using method thereof. The kit comprises lyophilized isothermal amplification agent, reconstitution solution, positive control dry powder, negative control, developing solution, lysate and confining liquid. A primer has good specificity and high sensitivity as well as extremely high reaction efficiency, the detection time can be greatly saved, the operation is simple, expensive instruments are not needed, and the lowest detection limit is up to 11 CFU/Test. The kit has the potential of conducting real-time monitoring, and is more convenient to transport, thus providing convenience for the base detection work of remote regions. The kit can be widely applied to laboratories and on-site rapid detection in the fields of food, beverage, drinking water and the like, and has wide application prospects and good economic and social benefits.