The invention provides a wing flexibility matrix measurement device. The wing flexibility matrix measurement device comprises a deformation measurement device, a model supporting device, a work platform, a mobile device, a force application mechanism and a numerical control positioning system, wherein the mobile device is arranged on the work platform, the force application mechanism is arranged on the mobile device, the numerical control positioning system is connected with the mobile device and the force application mechanism, the model supporting device is fixed at an end portion of the work platform, the mobile device is controlled through the numerical control positioning system for moving on the work platform so as to drive the force application mechanism to move, the force application mechanism is controlled for applying pressure at different positions of a wing, the deformation measurement device is fixed at a position close to the work platform for measuring deformation of the wing. The wing flexibility matrix measurement device is advantaged in that the structure is simple, accurate measurement is realized, a measurement process is easy to operate, accuracy of wing flexibility matrix measurement is improved, and workload of workers is reduced.