The invention discloses a centering verification method for an internal penetrating type eddy current detection probe. The centering verification method comprises the following steps of 1, defining the number n of centering measuring points; 2, processing through holes in a calibrating tube according to the number n of the measuring points determined in the step 1, enabling the through holes to be peripherally distributed along the calibrating tube at even degrees; 3, enabling the axial distribution of the through holes to be not less than the detection sensitivity of the probe; 4, setting the sampling frequency, the acquisition speed and the number of channels; 5, acquiring an eddy current signal of the whole calibrating tube, wherein the eddy current signal comprises a difference channel signal; 6, calibrating the eddy current signal acquired in the step 5, wherein the calibrating comprises phase position normalization and amplitude normalization; and 7, choosing the difference channel signal in the eddy current signal acquired in the step 5, measuring the eddy current signal of each through hole in the eddy current signal of the whole calibrating tube, and determining the centering of the probe.