The invention discloses an identification method of herba patriniae in a Chinese herbal compound, and particularly relates to a thin-layer identification method of herba patriniae in a Chinese herbal compound. The identification method comprises the following steps of preparing a reference crude drug solution; preparing a test product solution; preparing a negative sample solution; performing sample application on the same silica gel G thin-layer plate with a capillary sample applicator; placing the thin-layer plate into a chromatographic jar, performing expansion with an expanding agent, taking out and drying the thin-layer plate in air; and inspecting a thin-layer chromatographic sheet to obtain a result for identification, wherein a main spot of the same color is developed at a position, which corresponds to a reference crude drug chromatography, on a test product chromatography, but no spot is developed on a negative sample. The identification method disclosed by the invention is simple in technology, good in separation effect and extremely high in specificity; and the accuracy of component identification of herba patriniae in the Chinese herbal compound is further improved, and the identification method has a huge popularization and application potential in the field of identification of herba patriniae-related medicines.