The invention relates to a method for enhancing the demulsifying property of demulsifying bacteria. The method includes the following steps that (1) Fe3O4 magnetic nanoparticles are prepared through a coprecipitation method, and the obtained Fe3O4 magnetic nanoparticles are modified so that hydrophobic magnetic nanoparticles or charged magnetic nanoparticles can be obtained; (2) biological demulsifying bacteria are cultivated to obtain bacterium suspension; (3) one of the hydrophobic magnetic nanoparticles or charged magnetic nanoparticles and the bacterium suspension are mixed according to the mass ratio of (0.1-0.4):1, so that magnetic response demulsifying bacteria with the high demulsifying property are obtained; and (4) demulsification of the magnetic response demulsifying bacteria is facilitated under the external magnetic field action. Compared with the prior art, the demulsifying property of the bacteria is enhanced, the demulsifying rate of the bacteria is increased, and dehydration quality of the bacteria is improved.