The invention discloses a dispensing production line of ceramic substrates. The dispensing production line comprises a conveying roller line, jacking and locating mechanisms, a movable spray gun assembly and a control mechanism, wherein the movable spray gun assembly is above the conveying roller line, and is used for performing dispensing operation on the ceramic substrates; the jacking and locating mechanisms are below the conveying roller line; the control mechanism is fixed to the conveying roller line; the conveying roller line comprises a bracket; the bracket comprises a bottom plate and supporting legs; the supporting legs are fixedly mounted on the bottom plate; buffer ground feet are arranged at the four corners of the lower part of the bottom plate; a roller frame is fixedly mounted at the upper part of the bracket; and rollers are rotatably connected on the roller frame through rotating shafts. According to the dispensing production line, the jacking and locating mechanisms rise to locate the ceramic substrates on the conveying roller line under the condition that the operation of the conveying roller line is not affected, the dispensing operation is facilitated, the structure is simple and easy to operate, and the dispensing process does not affect conveying of the ceramic substrates.