The invention discloses a method for extraction of chlorophyll from safflower seedlings. The method includes: conducting root removal operation on fresh safflower seedlings, in a protective atmosphere of inert gas, squeezing the root removed fresh safflower seedlings by vacuum negative pressure method to obtain juice and residual, filtering the juice, combining the filter residue obtained by filtration and the residual to obtain a to-be-extracted material; extracting the to-be-extracted material by soaking and microwave assisted ultrasonic wave extraction method to obtain a filtered liquid; filtering the filtered liquid, adsorbing the filtered liquid with macroporous resin, and collecting chlorophyll-rich eluent; and carrying out centrifugation, separation and concentration as well as three-stage reverse osmosis concentration on the chlorophyll-rich eluent to obtain an extract, and drying the extract by lyophilization, thus obtaining chlorophyll powder. The method for extraction of chlorophyll from safflower seedlings provided by the invention has the advantages of high yield, low cost, low energy consumption, high purity and good color.