The invention provides a Zika virus antigen and application thereof, and belongs to the technical field of protein engineering. DNA sequences corresponding to a protein sequence determined through DNA analysis and protein structure analysis are synthesized, Nde I and Xho I cleavage sites are introduced, a commercialized pET30a plasmid serves as a basic carrier, pZE400 is built, the protein sequence is as shown in SEQ ID NO.1, and the DNA sequences are as shown in SEQ ID NO.2. A protein C end is labeled with a protein band HIS expressed by the expression carrier and Zika virus protein is obtained through affinity chromatography purifying and protein renaturation. The protein is good in immunogenicity and high in specificity, can be used for preparing a Zika virus detection test strip or kit, can also be used for preparing Zika virus vaccines, and is remarkable in market value.