The invention provides a kit for culturing pancreas stem cells. The kit comprises a preserving solution, a culture solution and a culture solution additive, wherein the preserving solution is an L-DMEM culture medium aqueous solution containing 5-10mug/mL of double antibody; the double antibody is a mixture of penicillin and streptomycin in weight ratio of 1:1; and the culture solution is an L-DMEM culture medium aqueous solution. The kit is used for integrally isolated culture, amplification, passage and cryopreservation of the pancreas stem cells. The kit can maintain versatility and surface property while reducing batch difference, can save time and energy and can quickly acquire a large quantity of pancreas stem cells.