The invention relates to a thin layer chromatographic analysis method for detection of flavonoids in polygonum viviparum. The method comprises the following steps: (a) preparation of a test sample solution: carrying out hydrolysis extraction of a polygonum viviparum medicinal material with hydrochloric acid methanol, to be used as the polygonum polygonum test sample solution; (b) preparation of reference substance solutions: respectively dissolving three reference substances of quercetin, isorhamnetin and kaempferol by methanol, to obtain the reference substance solutions; and (c) thin layer chromatography detection: with use of a silica gel thin layer chromatography plate, spreading the test sample and the reference substance solutions with a dichloromethane-acetone-methanol-formic acid system as a spreading agent; and spraying an AlCl3 chromogenic solution, and carrying out visual inspection of fluorescent thin layer chromatographic spots under an ultraviolet lamp. The method has the advantages of high efficiency, low cost and high practical value, and provides an effective way for identification and quality evaluation of the polygonum viviparum medicinal materials.